A technology of microbial flocculant and glycoprotein, which is applied in the field of water treatment, can solve the problems of environmental secondary pollution, etc., and achieve the effect of simplifying the adding process, reducing the content, and simplifying the adding process
Active Publication Date: 2015-12-09
XI'AN UNIVERSITY OF ARCHITECTURE AND TECHNOLOGY
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AI-Extracted Technical Summary
Problems solved by technology
[0003] Based on the problems existing in the prior art, the present invention provides a method for preparing a cationic modified glycoprotein microbial flocculant, which improves the activity of the glycoprotein microbial flocculant and solves the need for using the glycoprotein microbial flocculant in the prior art Coagulation aids ca...
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Abstract
The invention provides a preparation method and application of a cation-modified glycoprotein type microorganism flocculating agent. The preparation method comprises the following steps: mixing 3-chlorine-2-hydroxypropyl PDMC with a NaOH solution for reaction, adding a glycoprotein type microorganism flocculating agent for mixing, stirring under room temperature for 0.5-2 hours, and reacting under the temperature of 60-90 DEG C for 1-3 hours, thus obtaining the cation-modified glycoprotein type microorganism flocculating agent after purification. The flocculating agent is applied to removal of kaoline from wastewater. The cation-modified glycoprotein type microorganism flocculating agent prepared by the preparation method can effectively cationize a microorganism flocculating agent loaded with negative electricity, so that a flocculating aid used in a microorganism flocculating agent use process is omitted. In the use process, other flocculating aids are not needed, so that suspensions in kaoline simulation wastewater can be removed; therefore the adding process of the flocculating agent is simplified. The method disclosed by the invention finally achieves the effect of simplifying the flocculating agent adding technology, so that the content of inorganic flocculating acid substances in settled sludge is reduced, and secondary pollution caused by the settled sludge to the environment is alleviated.
Application Domain
Biological water/sewage treatment
Technology Topic
GlycoproteinChemistry
+7
Examples
- Experimental program(12)
- Comparison scheme(5)
- Effect test(6)
Example Embodiment
[0030] Example 1:
[0031] This embodiment provides a method for preparing a cationic modified glycoprotein microbial flocculant. The method includes the following steps:
[0032] Step 1: Preparation of glycoprotein microbial flocculant:
[0033] Step 1.1, prepare seed liquid:
[0034] Pick a ring of bacteria from the Klebsiella strain NY1 preservation slant, insert it into the beef extract peptone liquid medium and cultivate to obtain the OD 600nm Klebsiella NY1 seed liquid of 1.82±0.08;
[0035] Step 1.2, Klebsiella NY1 fermented to produce flocculant:
[0036] Sterilize 100 mL of fermentation medium at 121°C for 30 min. In a sterile environment, draw 2 mL of Klebsiella NY1 seed liquid and add it to the fermentation medium. At 30℃, 160r/min, constant temperature shaking culture for 72h to obtain crude glycoprotein microbial flocculant;
[0037] Step 1.3, the purification method of the crude product of glycoprotein microbial flocculant:
[0038] Take the fermentation broth after 72h, centrifuge at 10000r/min, 4℃ for 15min to sterilize, add stabilizer sodium chloride to the supernatant, add 0.5g~2g of stabilizer to each 100mL supernatant, stir well After mixing with absolute ethanol, the precipitate was precipitated, the supernatant was decanted, and the supernatant was centrifuged at 10000r/min at 4°C for 1 min. The obtained solid precipitate was washed three times with a small amount of absolute ethanol and dried at 45°C to obtain glycoprotein The pure product of similar microbial flocculant.
[0039] Step 2: Cationic modification of glycoprotein microbial flocculant:
[0040] Mix 0.015mol of 3-chloro-2-hydroxypropyltrimethylammonium chloride (CTA) with 1mol/L NaOH solution at a molar ratio of 1:1, let it react for 10 minutes, add 10g of NY1 Produce pure flocculant. The mixture was stirred at room temperature for 0.5 h, and placed in a thermostat to react at 60° C. for 1 hour to obtain a mixture of cationic modified glycoprotein microbial flocculants.
[0041] Step 3: Purification of cationized glycoprotein microbial flocculant:
[0042] Completely dissolve the mixture obtained in step 2 above in 10mL of water, then add 30mL of absolute ethanol to precipitate the precipitate, filter, wash the precipitate three times, discard the supernatant, and keep the precipitate at 10000r/min, 4℃ After centrifugation for 1 min and drying, the purified cationic modified glycoprotein microbial flocculant was obtained, and the Zeta potential was measured.
Example
[0055] Application comparative example 1-1:
[0056] This application example provides a method for removing kaolin in wastewater, which uses the pure glycoprotein microbial flocculant prepared in Comparative Example 1.
[0057] Combine 0.4mL glycoprotein microbial flocculant (2g/L) and 0.2mL of 1mol/L coagulant aid CaCl 2 The solution was sequentially added to 50 mL of 2g/L kaolin wastewater suspension (pH 7.5), stirred rapidly for 1 min, stirred slowly for 2 min, and allowed to stand for 5 min. The absorbance of the supernatant at 550 nm was measured. Calculate the flocculation rate of the sample.
Example
[0058] Application Comparative Example 1-2:
[0059] This application example provides a method for removing kaolin in wastewater. The method adopts the purified cationic modified glycoprotein microbial flocculant prepared by the preparation method in Example 1.
[0060] Combine 0.4mL of purified cationic modified glycoprotein microbial flocculant (4g/L) and 0.2mL of 1mol/L coagulant aid CaCl 2 The solution was added to 50 mL of kaolin wastewater suspension (pH 7.5) with a concentration of 2g/L, stirred rapidly for 1 min, stirred slowly for 2 min, and allowed to stand for 5 min. The absorbance of the supernatant at 550 nm was measured. Calculate the flocculation rate of the sample.
[0061] Result test characterization:
[0062] The Zeta potential of the glycoprotein microbial flocculant before cationization in Comparative Example 1 was -49.7 mv, while the Zeta potential of the cation-modified glycoprotein microbial flocculant in Example 1 was +12.3 mv; Comparative Example 1 was applied The flocculation rate of the glycoprotein microbial flocculant before cationization in -1 was 89.2%, and the flocculation rate of the glycoprotein microbial flocculant after cationization in Example 1 was 92.3%. Application of Comparative Example 1-2 Although a coagulant aid was added to the flocculant of Example 1, the flocculation rate of the cationized glycoprotein microbial flocculant in Comparative Example 2 was 92.2%. Therefore, the example The cationic modified glycoprotein microbial flocculant prepared in 1 does not need to add a coagulant aid during use.
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PUM
| Property | Measurement | Unit |
| Concentration | 2.0 | g/l |
| tensile | MPa | |
| Particle size | Pa | |
| strength | 10 |
Description & Claims & Application Information
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FAQs
What are the examples of flocculating agents? ›
Aluminum sulfate or alum (Al2(SO4)3), ferric chloride (FeCl3) and ferric sulfate (Fe(SO4)3) are the most widely used flocculants [1, 70]. These flocculants have a long history of use in removing colloidal particles from water and wastewater [15, 17].
What are flocculating agents used for? ›Flocculating agents are chemical additives that cause suspended solids to form aggregates called flocs. These agents are used in water treatment, municipal and industrial waste treatment, mineral processing, and papermaking. Flocculating agents are either inorganic salts or water-soluble organic polymers.
What is the use of cationic flocculant? ›Cationic cellulose-based flocculants, hold positively charged groups, which are fundamental for the neutralization of negatively charged suspended dye molecules. Also, due to the relatively long chains of the polymers with medium charge densities, bridging between the particles will be a complementary mechanism.
What are flocculants how are they used to treat contaminated water? ›Flocculation is a water treatment process where solids form larger clusters, or flocs, to be removed from water. This process can happen spontaneously, or with the help of chemical agents. It is a common method of stormwater treatment, wastewater treatment, and in the purification of drinking water.
What is the best flocculating agent? ›Ferric sulfate (Fe(SO4)3)) is an iron-based salt that is one of the most widely used flocculants across different industries, along with alum and ferric chloride. It has slightly acidic properties that make it suitable for pH adjustment and is highly effective as a flocculant agent.
What are the three types of flocculation? ›concept of the flocculation mechanism has been classified into charge neutralization, electrostatic patch, and polymer bridging [63].
What are flocculants examples? ›The aluminum-based flocculants include aluminum sulfate, aluminum chloride, sodium aluminate, aluminum chlorohydrate, and polyaluminum chloride. The iron-based flocculants include ferric chloride, ferric sulfate, ferrous sulfate, and ferric chloride sulfate [15, 69].
What is an example of flocculation? ›One example is the natural compound extracted from the Opuntia ficus-indica cactus as a flocculation and concentration agent for bacteria suspended in water.
What is the flocculation method? ›Flocculation in water treatment is a chemical-based process in which chemical is added to the wastewater in sequence, allowing the tiny particles to collect together and form a large cluster or floc. In wastewater treatment, the whole flocculation process is carried out in steps.
Does flocculant remove bacteria? ›Coagulation, flocculation, and sedimentation processes could be a reliable treatment for bacterial removal because suspended, colloidal, and soluble particles can be removed.
What happens during flocculation? ›
Flocculation is the “snowballing” of small particles into larger particles (called “floc”). It is a time-dependent process that directly affects clarification efficiency by providing multiple opportunities for particles suspended in water to collide through gentle and prolonged agitation.
How well does flocculant work? ›The particles quickly coagulate together and sink to your pool floor in a matter of hours. Pool flocculant is very effective. Flocculant keeps particles clumped together until you remove them with a vacuum. No stragglers are left behind causing cloudy spots in your swimming pool water.
What pH level is needed for flocculant? ›The highest flocculating activity is achieved at pH 5.0 with a mean difference of about 86% from the lowest activity at pH 9.0, 10.0, and 11.0 (P > 0.05).
What is a natural flocculant? ›Natural flocculants are mainly obtained from chitosan, starch, cellulose, tannin, microbial raw materials and animal glue and gelatin.
What is one of the most common chemical flocculants used in water purification today? ›Aluminum sulfate (alum) is the most common coagulant used for water purification.
Does flocculant affect pH? ›Because flocculants can affect the pH level, you'll want to start out with it at about 7.0 so you can make sure your sanitizer keeps working at maximum efficiency.
Is flocculant hazardous? ›This material is not considered hazardous under the OSHA Hazard Communication Standard (HazCom 2012). None known. General advice : No hazards which require special first aid measures.
What are the advantages of flocculants? ›The benefit of a flocculant is that it can carry active groups with a charge which will counterbalance the charge of the particles, and adsorb on particles and cause destabilization either by bridging or charge neutralization.
Why is flocculation important in water treatment? ›Coagulation and flocculation are both critical processes to separate and remove suspended solids in water and wastewater treatment. These processes improve the clarity of the water to reduce turbidity.
What is another word for flocculation? ›- downy.
- fluffy.
- woolly.
What does flocculation remove? ›
Flocculants are lightweight, medium weight and heavy polymers that cause the destabilized clumps of particles to agglomerate and drop out of the solution, removing them from the filtered water.
What are flocculation chemicals? ›Flocculants, or flocculating agents, are chemicals that promote flocculation by causing colloids and other suspended particles in liquids to aggregate, forming a floc. Flocculants are used in water treatment processes to improve the sedimentation or filterability of small particles.
What type of chemical is a flocculant? ›Polymers are chemicals used in flocculation. Flocculation is the process of agglomerating destabilized particles into bigger flocs. In wastewater flocculation and sludge treatment, colloidal particles are flocked in order to aid their removal or to help sludge dewatering.
What is flocculation tests? ›A Flocculation Test Procedure is used to determine how much Flocculants need be added to solids in suspension (slurry or pulp) to cause the individual particles to collect in the form of flocs.
What is bacterial flocculation? ›Abstract. Microbial flocculation is a phenomenon of aggregation of dispersed bacterial cells in the form of flocs or flakes. In this study, the mechanism of spontaneous flocculation of Escherichia coli cells by overexpression of the bcsB gene was investigated.
What does too much flocculant look like? ›Too much flocculant means that it will start to stick to itself over and above the bacteria and algae spores that we're trying to clear. The issue with this happening is that it won't sink to the bottom. It'll stay floating in your pool and start to cause more problems, like clogging your pool filter.
Does flocculant dissolve? ›In contact with water, the flocculant dissolves and forms a viscous gel that is similar to hair gel. This gel then transforms the floor into a skating rink, which greatly increases the risk of falls. Due to their role, flocculants should be added in small proportions to a large quantity of water (less than 0.1%).
How long does the flocculation process take? ›Design contact times for flocculation range from 15 or 20 minutes to an hour or more, and flocculation requires careful attention to the mixing velocity and amount of mix energy. To prevent floc from tearing apart or shearing, the mixing velocity and energy are usually tapered off as the size of floc increases.
What does activated sludge remove? ›The activated sludge process is a means of treating both municipal and industrial wastewater. The activated sludge process is a multi-chamber reactor unit that uses highly concentrated microorganisms to degrade organics and remove nutrients from wastewater, producing quality effluent.
Does flocculant have a shelf life? ›“Emulsion” flocculants have a shelf life of between 6 and 18 months and should be stored between 50°F and 90°F in a tightly sealed container.
Can you overdose flocculant? ›
Under- dosing a traditional polymeric flocculant does not provide enough polymer to effectively bridge particles thus leading to incomplete clarification while overdos- ing results in restabilization of the suspension leading to elevated levels of residual polymer and higher tur- bidity.
Can you add shock after flocculant? ›It Should Not Be Done Together. While shocking and adding algaecide is effective in getting rid of algae, it should not be done together. This is because when you mix chlorine and algaecide together, it renders both of them useless.
Will flocculant work if pH is low? ›If your pool's pH is on the higher end (7.6 or above), you may need less flocculant as the higher pH will make the flocculation process more efficient. Conversely, if your pool's pH is on the lower end (below 7.4), you may need more flocculant as the lower pH can inhibit flocculation.
Does flocculant dissolve in water? ›Flocculants belong to the water soluble polymer class, and so they fully dissolve in water.
Should I shock my pool before using flocculant? ›Before using any water clarifier , you must treat any algae in your pool. No amount of water clarifier will eliminate algae in your water so you need to shock your pool first. If you have the early stages of algae growth, pool flocculant can help bind floating algae particles, making it easier to vacuum.
Is Vinegar A flocculant? ›Common flocculants include calcium chloride, vinegar and epsom salts.
Is Epsom salt a flocculant? ›Epsom Salt is one of the most popular and widely used ceramic glaze flocculants.
Does flocculant remove metals? ›As a result of its simplicity, dependability, and effectiveness, flocculation is widely used to remove heavy metals from water [3]. Traditional inorganic flocculants effectively remove colloidal substances, suspended particles, and turbidity from water, but it is more difficult to remove dissolved heavy metal ions [4].
Which are the 3 most widely used disinfectants in wastewater treatment? ›Some of the most commonly used disinfectants for decentralized applications include chlorine, iodine, and ultraviolet (UV) radiation. Wastewater must be adequately treated prior to disinfection in order for any disinfectant to be effective.
Which chemical is used most often to disinfect the water treatment system? ›Chlorine and chloramine are the major disinfectants used in public water systems.
What is natural flocculant for water treatment? ›
The findings of this study proved that pomegranate seed powder can be considered a promising, environmentally friendly and effective coagulant-flocculant agent for industrial wastewater treatment.
What is a simple example of flocculation? ›One example is the natural compound extracted from the Opuntia ficus-indica cactus as a flocculation and concentration agent for bacteria suspended in water.
What are 5 examples of suspending agents? ›- Dissolution.
- Surfactant.
- Alginic Acid.
- Povidone.
- Polysaccharide.
- Gum Tragacanth.
- Emulsifying Agent.
The flocculating agents are normally grouped into three types which are organic synthetic flocculants, inorganic flocculants and naturally occurring (Aljuboori et al., 2013). ...
Are electrolytes flocculating agents? ›Electrolytes are probably the most widely used flocculating agents. They act by reducing the electrical forces of repulsion between particles, thereby allowing the particles to form the loose flocs so characteristic of a flocculated suspension.
What is the most common flocculation? ›Aluminum sulfate or alum (Al2(SO4)3), ferric chloride (FeCl3) and ferric sulfate (Fe(SO4)3) are the most widely used flocculants [1, 70]. These flocculants have a long history of use in removing colloidal particles from water and wastewater [15, 17].
What are the different types of flocculation process? ›There are two types of flocculation : natural flocculation and chemical flocculation.
What chemical causes flocculation? ›Chemicals (coagulants) are added to the water to bring the nonsettling particles together into larger, heavier masses of solids called floc. Aluminum sulfate (alum) is the most common coagulant used for water purification. Other chemicals, such as ferric sulfate or sodium aluminate, may also be used.