Epinephrine/Norepinephrine ELISA Kit (Colorimetric) (2022)

Epinephrine/Norepinephrine ELISA Kit (Colorimetric) Summary

Description

Epinephrine/Norepinephrine ELISA Kit is an enzyme immunoassay for the quantitative determination of Epinephrine and Norepinephrine in plasma and urine. This product contains two assay plates, for Epinephrine and Normetanephrine separately.

Standard Curve Range

Adrenaline: 0.7 to 200 ng/mL in Urine; 18 to 6667 pg/mL in Plasma, Noradrenaline: 2.5 to 1000 ng/mL in Urine; 93 to 33333 pg/mL in Plasma

Sensitivity

Adrenaline: 0.9 ng/mL Urine, 10 pg/mL Plasma
Noradrenaline: 1.7 ng/mL Urine, 36 pg/mL Plasma

Assay Type

Quantification

Inter-Assay

Epinephrine: 15.6%, Norepinephrine: 11.75%

Intra-Assay

Epinephrine: 12.57%, Norepinephrine: 12.95%

Spike Recovery

Epinephrine: 107% in Urine, 92% in PlasmaNorepinephrine: 109% in Urine, 97% in Plasma

Sample Volume

Adrenaline ELISA : 100 uL (extracted)
Noradrenaline ELISA : 20 uL (extracted)

Kit Type

ELISA Kit (Colorimetric)

Applications/Dilutions

Dilutions
  • ELISA
  • Quantification
Application Notes

Useful for quantification.

Publications
Read Publications using
KA1877 in the following applications:
  • ELISA

    2 publications

Packaging, Storage & Formulations

Storage

Store at 4C.

Kit Components

Components
  1. Acylation Buffer: Ready to use. Buffer with light alkaline pH for the acylation.
  2. Acylation Reagent: Ready to use. Acylation reagent in DMF and DMSO.
  3. Adhesive Foil: Ready to use. Adhesive foils in a resealable pouch.
  4. Adjustment Buffer: Ready to use, TRIS buffer.
  5. Adrenaline Antiserum: Ready to use, rabbit anti-adrenaline antibody, blue coloured.
  6. Adrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in aresealabel blue pouch with desiccant.
  7. Assay Buffer: Ready to use, 1 M hydrochloric acid and a non-mercury preservative.
  8. Coenzyme: Ready to use, S-adenosyl-L-methionine.
  9. Enzyme Conjugate: Ready to use, goat anti-rabbit immunoglobulins, conjugated withperoxidase.
  10. Enzyme: Lyophilized Catechol-O-methyltransferase.
  11. Extraction Buffer: Ready to use, buffer containing carbonate.
  12. Extraction Plate: Ready to use, coated with boronate affinity gel in a resealabel pouch.
  13. Hazards identification:H225 Highly flammable liquid and vapour.H360 May damage fertility or the unborn child.H319 Causes serious eye irritation
  14. Hydrochloric Acid: Ready to use, 0.025 M hydrochloric acid, yellow coloured.
  15. Noradrenaline Antiserum: Ready to use, rabbit anti-noradrenaline antibody, yellowcoloured.
  16. Noradrenaline Microtiter Strips: Ready to use, antigen precoated microwell plate in aresealable yellow pouch with desiccant.
  17. Stop Solution: Ready to use, 0.25 M sulfuric acid.Hazards identification:H290 May be corrosive to metals.H314 Causes severe skin burns and eye damage.
  18. Substrate: Ready to use, chromogenic substrate containing tetramethylbenzidine,substrate buffer and hydrogen peroxide.
  19. Wash Buffer Concentrate: 50x concentrated buffer with a non-ionic detergent andphysiological pH.

Notes

Quality control test: Standard curve.

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Epinephrine/Norepinephrine ELISA Kit (Colorimetric)

  • adrenaline
  • Arterenol
  • l-Adrenaline
  • L-epinephrine
  • Levarterenol
  • Levoepinephrine
  • L-Noradrenaline
  • L-Norepinephrine
  • noradrenaline

Background

Adrenaline (epinephrine) and noradrenaline (norepinephrine) are extracted by using a cis-diol-specific affinity gel, acylated and then converted enzymatically. The competitive ELISA kit uses the microtiter plate format. The antigen is bound to the solid phase of the microtiter plate. The derivatized standards, controls and samples and the solid phase bound analytes compete for a fixed number of antibody binding sites. After the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Quantification of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standard concentrations.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Epinephrine/Norepinephrine ELISA Kit (KA1877)(28)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: ELISA.

Filter By Application

ELISA

(2)

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Filter By Species

Rat

(1)

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Showing Publications 1 - 10 of 28. Show All 28 Publications.

Publications using KA1877 Applications Species
Sudo H, Tsuji AB, Sugyo A et al. Preclinical Evaluation of the Acute Radiotoxicity of the alpha -Emitting Molecular-Targeted Therapeutic Agent 211At-MABG for the Treatment of Malignant Pheochromocytoma in Normal Mice. Transl Oncol. 2019 May 08 [PMID: 31078058]
Rao X, Asico LD, Zanos P et al. Alpha2B-Adrenergic Receptor Overexpression in the Brain Potentiate Air Pollution-induced Behavior and Blood Pressure Changes. Toxicol Sci. 2019 May 01 [PMID: 30812033]
Kliszczewicz B, Bechke E, Williamson C et al. Citrus Aurantium and caffeine complex versus placebo on biomarkers of metabolism: a double blind crossover design. J Int Soc Sports Nutr. 2019 Feb 06 [PMID: 30728061]
Kim JA, Ahn HJ, Yang M et al. Intraoperative use of dexmedetomidine for the prevention of emergence agitation and postoperative delirium in thoracic surgery: a randomized-controlled trial. Can J Anaesth. 2019 Jan 24 [PMID: 30710258]
Hashimoto O, Funaba M, Sekiyama K et al. Activin E Controls Energy Homeostasis in Both Brown and White Adipose Tissues as a Hepatokine. Cell Rep. 2018 Oct 30 [PMID: 30380411]
Ribeiro-da-Silva M, Vasconcelos DM, Alencastre IS et al. Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement. Sci Rep. 2018 Oct 30 [PMID: 30375409]
Boehme J, Le Moan N, Kameny RJ et al. Preservation of myocardial contractility during acute hypoxia with OMX-CV, a novel oxygen delivery biotherapeutic. PLoS Biol. 2018 Oct 18 [PMID: 30335746]
Kirino K, Nakahata T, Taguchi T et al. Efficient derivation of sympathetic neurons from human pluripotent stem cells with a defined condition. Sci Rep Aug 27 2018 [PMID: 30150715]
Vasamsetti SB, Florentin J, Coppin E et al. Sympathetic Neuronal Activation Triggers Myeloid Progenitor Proliferation and Differentiation. Immunity Jun 26 2018 [PMID: 29958804]
Loftus TJ, Mira JC, Miller ES et al. The Post-injury Inflammatory State and the Bone Marrow Response to Anemia. Am J Respir Crit Care Med May 16 2018 [PMID: 29768025]
Xi P, Du J, Liang H et al. Intraventricular Injection of LKB1 Inhibits the Formation of Diet-Induced Obesity in Rats by Activating the AMPK-POMC Neurons-Sympathetic Nervous System Axis Cell. Physiol. Biochem. May 9 2018 [PMID: 29763913] (ELISA) ELISA
Jackson DA, Michael T, Vieira de Abreu A et al. Prevention of Severe Hypoglycemia-Induced Brain Damage and Cognitive Impairment with Verapamil. Diabetes May 3 2018 [PMID: 29724724]
Macias D, Cowburn AS, Torres-Torrelo H et al. HIF-2? is essential for carotid body development and function. Elife Apr 19 2018 [PMID: 29671738]
Dhar P, Sharma VK, Das SK et al. Differential responses of autonomic function in sea level residents, acclimatized lowlanders at >3500 m and Himalayan high altitude natives at >3500 m: A cross-sectional study. Respir Physiol Neurobiol Apr 9 2018 [PMID: 29649580]
Kushnir A, Santulli G, Reiken SR et al. Ryanodine Receptor Calcium Leak in Circulating B-Lymphocytes as a Biomarker in Heart Failure. Circulation Mar 28 2018 [PMID: 29593014]
Zhu RT, Liu XH, Shi YW et al. Propranolol can induce PTSD-like memory impairments in rats. Brain Behav Jan 18 2018 [PMID: 29484264]
Perry RJ, Wang Y, Cline GW et al. Leptin Mediates a Glucose-Fatty Acid Cycle to Maintain Glucose Homeostasis in Starvation. Cell Jan 4 2018 [PMID: 29307489]
Hu B, Zhang J, Wang J et al. Responses of PKCepsilon to cardiac overloads on myocardial sympathetic innervation and NET expression. Auton Neurosci 2018 Mar [PMID: 29195789] (Rat) Rat
Bubak MP, Heesch MWS, Shute RJ et al. Irisin and Fibronectin Type III Domain-Containing 5 Responses to Exercise in Different Environmental Conditions. Int J Exerc Sci Sep 1 2017 [PMID: 28966707]
Samad MB, Mohsin MNAB, Razu BA et al. [6]-Gingerol, from Zingiber officinale, potentiates GLP-1 mediated glucose-stimulated insulin secretion pathway in pancreatic B-cells and increases RAB8/RAB10-regulated membrane presentation of GLUT4 transporters in skeletal muscle to improve hyperglycemi BMC Complement Altern Med 2017 Aug 9 [PMID: 28793909]
Zhang LC, Huang Z, Li PB et al. Diazoxide protects rat vascular endothelial cells against hypoxia and cold-induced damage. Exp Ther Med 2017 May 5 [PMID: 28587398]
Perry RJ, Peng L, Abulizi A et al. Mechanism for leptin's acute insulin-independent effect to reverse diabetic ketoacidosis. J Clin Invest 2017 Feb 1 [PMID: 28112679]
Sinclair M, Keeley T, Lefebvre AC, Phillips CJ Behavioral and Physiological Responses of Calves to Marshalling and Roping in a Simulated Rodeo Event. Animals (Basel) 2016 Apr 28 [PMID: 27136590]
White CW, Lillico R, Sandha J et al. Physiologic Changes in the Heart Following Cessation of Mechanical Ventilation in a Porcine Model of Donation After Circulatory Death: Implications for Cardiac Transplantation. Am J Transplant 2016 Mar [PMID: 26663659]
Brain KL, Allison BJ, Niu Y et al. Induction of controlled hypoxic pregnancy in large mammalian species. Physiol Rep 2015 Dec [PMID: 26660546] (ELISA) ELISA
Tsuda H, Kotani T, Sumigama S et al. Impact of warning bleeding on the cortisol level in the fetus and neonatal RDS/TTN in cases of placenta previa. J Matern Fetal Neonatal Med 2015 Jun [PMID: 25001429]
Kwon BS, Ha CM, Yu S et al. Microscopic nodes and ducts inside lymphatics and on the surface of internal organs are rich in granulocytes and secretory granules. Cytokine. 2012 Aug 09 [PMID: 22884518]
Wang Y, Nasiri AR, Damsky WE et al. Uncoupling Hepatic Oxidative Phosphorylation Reduces Tumor Growth in Two Murine Models of Colon Cancer. Cell Rep Jul 3 2018 [PMID: 9972790]
Show All 28 Publications.

Reviews for Epinephrine/Norepinephrine ELISA Kit (KA1877) (0)

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

  • ELISA
  • View all Protocols, Troubleshooting, Illustrated assays and Webinars.

FAQs for Epinephrine/Norepinephrine ELISA Kit (KA1877). (Showing 1 - 5 of 5 FAQs).

  1. Can it be used with the samples from sheep?
    • The KA1877 was developed and validated for the use of human samples. Since Epinephrine, Norepinephrine are brain derived chemicals, these targets are not species specific, theoretically all kinds of species can be used. Meaning, this kit should work for whatever species as long as the target exists in the species systems.
  2. Can it be used with tissue homogenates?
    • This kit was not validated for tissue homogenates, so if this customer would like to test it, we cannot guarantee the kit will work.
  3. Is this assay for use in rat plasma? The species is not listed.
    • The kit should work for any species as the target should be the same across all species.
  4. Can this kit be used with rat serum/plasma?
    • Rat serum and plasma samples can be tested with the KA1894, KA1891 and KA1877. There is no species cross-reactivity between rat samples and the antisera of our assays.
  5. Hi! I'm interested in your nor/epi elisa kit (KA1877). However, I have urine and serum samples. I only see plasma and urine listed as suitable sample types, so wanted to confirm whether serum could possibly be used
    • This assay has not been validated for serum as serum is not the sample of choice for catecholamine determination. This does not mean that serum cannot be measured, just that for clinical use this does not make sense. Due to the extraction system prior to the ELISA many types of samples could be used, including serum. In summary: for clinical purposes EDTA plasma is the sample of choice. For research purposes serum could be used, but it is up to the researcher to find out if catecholamine determination in serum will provide the required information.

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