Attachment and phagocytosis by salmon macrophages of agarose beads coated with human C3b and C3bi (2023)

Cited by (35)

  • An overview of complement systems in teleosts

    2022, Developmental and Comparative Immunology

    Citation Excerpt :

    CR1 in circulating cells acts as an immune adherence receptor to facilitate the removal of C3b/C4b-opsonized immune complexes or pathogens, and CR1 has cofactor activity for CFI-mediated cleavage of C3b to iC3b and further cleavage to C3c and C3dg (Noris and Remuzzi, 2013). In teleosts, CR3 has been cloned from channel catfish and rainbow trout (Qian et al., 1999; Mikrou et al., 2009), and C3-opsonized particles have been shown to increase phagocytic activity of macrophages and neutrophils in several teleost species (Johnson and Smith, 1984; Matsuyama et al., 1992). In addition, it has been shown that teleost complement and Ig have a synergistic effect on the opsonization of microbes (Olivier et al., 1986; Jenkins and Ourth, 1993).

    Complement plays an important role in the innate immune system, and it comprises about 35 individual proteins. In mammals, complement is activated via three different pathways, the classical pathway, the alternative pathway, and the lectin pathway. All three activation pathways produce C3-convertase in different forms. C3-convertase cleaves C3 to C3a and C3b and initiates a cascade of cleavage and activation, eventually resulting in the formation of the membrane attack complex. Complement activation results in the generation of activated fragments that are involved in microbial killing, phagocytosis, inflammatory reactions, immune complex clearance, and antibody production. Although the complement system has been studied extensively in mammals, complement is less well understood in teleosts. This review summarizes the current knowledge of the teleost complement components involved in phagocytosis, chemotaxis, and cell lysis. We report the characterized complement components in various teleost species. In addition, we provide a comprehensive compilation of complement regulators, and this information is used to analyze the role of complement regulators in pathogen infection. The influence of complement receptors on the immune responses of teleosts is reviewed. Finally, we propose directions for future study of the molecular evolution, structure, and function of complement components in teleosts. This review provides new insights into the complement system of recognition and defense, and such knowledge is essential for the development of new immune strategies in aquaculture.

  • A pathway-focused RT-qPCR array study on immune relevant genes in rainbow trout (Oncorhynchus mykiss) harboring cecropin P1 transgene

    2019, Fish and Shellfish Immunology

    Citation Excerpt :

    Later, complement activities, which were implicated in the inactivation of lethal toxicities in rainbow trout, was reported by the same group [50]. Additionally, Johnson et al. [51], observed that complement receptor mediated phagocytic engulfing of complement components (C3b/C3bi) coated beads, in vitro, into salmon macrophages. Since complement cascades play important roles in fish immunity, especially in mediating phagocytosis, it agrees with our results of RT-qPCR array that consistent up-regulation of binding ligand (mbl2) and its down-stream complement components (c4, c6 and c7) were observed in the spleen of cecropin P1 transgenic rainbow trout.

    Recently, our laboratory had produced five families of transgenic rainbow trout harboring cecropin P1 transgene, and via repeated challenge studies these fish exhibited a significant elevation of resistance to infection by microbial pathogens. By cDNA microarray and mRNA deep sequencing (mRNA-seq) analyses on two of the five families of cecropin P1 transgenic fish, differentially expressed genes (DEGs) relevant to the innate and adaptive immune pathways in three different immune-related tissues, (i.e. spleen, kidney and liver) were profiled. These results supported our hypothesis that in addition to its direct microbicidal activity, the transgene product of cecropin P1 induces immunomodulatory activity in the transgenic host. Here, we have adapted the technique of quantitative reverse transcription real time PCR (RT-qPCR) array to analyze the expression of genes relevant to the innate and adaptive immune pathways in the rest three families. A RT-qPCR array was constructed with oligonucleotide primers of fifty-two innate/adaptive immune relevant DEGs shown to be the most perturbed by cecropin P1 transgene product in previous studies. Messenger RNA isolated from the spleen, kidney and liver of transgenic fish and non-transgenic fish control were studied on this array. Results of RT-qPCR array revealed that statistically significant perturbations of gene expression were detected in pathways of cytokine/chemokine signaling, Toll-like receptor signaling, complement cascade, antigen processing/presentation, lysosomal phagocytosis and leukocyte trans-endothelial migration in the transgenic spleen; extracellular matrix (ECM) organization and leukocyte trans-endothelial migration pathways in the transgenic kidney; lysosomal activity pathway in the transgenic liver. Furthermore, genes related to the pathways of the peroxisome proliferator-activated receptors (PPAR) signaling, lipid metabolism process and arachidonic acid metabolism were also impacted in the transgenic liver. Findings of the current study are in good agreement with those discoveries in previous two transgenic families by cDNA microarray and mRNA-seq analyses.

  • Polyclonal anti-Candida antibody improves phagocytosis and overall outcome in zebrafish model of disseminated candidiasis

    2017, Developmental and Comparative Immunology

    Citation Excerpt :

    Complement- and antibody-mediated phagocytosis by macrophages has also been demonstrated in teleosts (Leiro et al., 1996, 2008). Intriguingly, opsonization with human serum also enhances phagocytosis of beads by salmon macrophages in a process dependent on complement deposition (Johnson and Smith, 1984). Although an Fc gamma receptor homolog has not yet been found in zebrafish, expansions of related receptors may allow for a more broad-specificity phagocytosis of antibody-opsonized targets (Akula et al., 2014).

    Fungal infections are a major cause of animal and plant morbidity and mortality worldwide. Effective biological therapeutics could complement current antifungal drugs, but understanding of their invivo mechanisms has been hampered by technical barriers to intravital imaging of host-pathogen interactions. Here we characterize the fungal infection of zebrafish as a model to understand the mechanism-of-action for biological antifungal therapeutics through intravital imaging of these transparent animals. We find that non-specific human IgG enhances phagocytosis by zebrafish phagocytes invivo. Polyclonal anti-Candida antibodies enhance containment of fungi invivo and promote survival. Analysis suggests that early phagocytic containment is a strong prognostic indicator for overall survival. Although polyclonal anti-Candida antibodies protect against disease, this is not necessarily the case for individual monoclonal anti-Candida antibodies. Thus, the zebrafish appears to provide a useful model host for testing if a biological therapeutic promotes phagocytosis invivo and enhances protection against candidemia.

  • A complement receptor for opsonized immune complexes on erythrocytes from Oncorhynchus mykiss but not Ictalarus punctatus

    2006, Molecular Immunology

    Immune complexes activate the classical pathway of complement resulting in the covalent deposition of fragments of the third (C3b) and fourth (C4b) components of complement, thus opsonizing the complexes for uptake by CD35 found on human erythrocytes. The complexes are then transported to and cleared from the circulation by the reticuloendothelial system. It has been shown that rainbow trout can remove immune complexes from the circulation in a complement-dependent manner similar to that found in the human. However, the cell or cell types involved have not been identified. The purpose of this study was to investigate whether a complement-dependent immune adherence receptor is expressed on erythrocytes from the rainbow trout (Oncorhynchus mykiss) and the channel catfish (Ictalarus punctatus). Coating fluorescent microparticles with BSA, and then binding them to anti-BSA created an artificial immune complex that was incubated with normal fish serum, normal human serum or EDTA-treated serum. The complement-coated immune complexes were then incubated with either fish or human erythrocytes and analyzed for binding by flow cytometry and further visualized by fluorescence microscopy. Our results indicate that erythrocytes from rainbow trout are capable of binding immune complexes when pretreated with serum from either the trout or human, but not when pretreated with serum containing EDTA. By contrast, erythrocytes from the channel catfish did not bind immune complexes pretreated with autologous or human serum. These data suggest that differences exist in receptor distribution between two closely related species of fish, and a potentially homologous relationship in receptor expression, and possibility function, exist between two highly divergent species.

  • A complement C3 fragment equivalent to mammalian C3d from the common carp (Cyprinus carpio): Generation in serum after activation of the alternative pathway and detection of its receptor on the lymphocyte surface

    2004, Fish and Shellfish Immunology

    A terminal degradation product (C3d) of mammalian complement component C3 plays an important role in modulation of the adaptive immune response through the interaction with complement receptor type 2 (CR2) on B cells. The present study is aimed at determining whether this is a functional bridge between the innate and adaptive immune systems in bony fish. The fragmentation of the complement component C3 in carp (Cyprinus carpio) serum, activated with zymosan, was analysed to ascertain if carp C3 also generates a mammalian C3d-like fragment under physiological conditions. A 35 kDa peptide reactive to the anti-carp C3 α-chain was detected on the zymosan particles and in the activated serum. Its N-terminal amino acid sequence identified it as carp C3d derived from the C3-H1 isoform. Another C3 isoform, C3-S, of carp was found to yield a C3d fragment at lower efficiency than C3-H1. Recombinant C3d fragments derived from C3-H1 and C3-S were produced in Escherichia coli as fusion proteins with glutathione-S-transferase (GST), and used for ligands to examine the presence of a possible CR2-like C3d receptor on carp lymphocytes. An enzyme-linked immunoadsorbent assay system, using the recombinant C3d proteins and anti-GST on a microplate to which was attached carp peripheral lymphocytes, detected a significant binding of carp C3d to the lymphocyte. The degree of binding of C3-H1-derived C3d was higher than that of C3-S-derived C3d. In addition, the binding of both ligands was inhibited by anti-C3 α-chain, but not by EDTA or EGTA, indicating that the putative C3d receptor does not require divalent cation. These properties agree well with those reported for mammalian CR2.

  • The isolation and culture of microglia-like cells from the goldfish brain

    2003, Journal of Neuroscience Methods

    Citation Excerpt :

    In addition, the uptake of DiIacLDL by the teleost cells indicates that they express scavenger receptors, which have also been implicated in myelin phagocytosis (Bell et al., 1994; DaCosta et al., 1997; Giulian and Baker, 1986; Mosley and Cuzner, 1996). Finally, it should be noted that the CR3 complement receptor has repeatedly been shown to mediate myelin phagocytosis in mammalian cells (Brücke and Friede, 1990; Mosley and Cuzner, 1996; Van der Laan et al., 1996) and several workers have demonstrated opsonic effects of C3 fragments on phagocytosis by fish immune cells (Johnson and Smith, 1984; Matsuyama et al., 1992). In addition, the CD18 component of the C3 receptor has recently been identified in the catfish and shown to be expressed on phagocytic cells (Qian et al., 1999).

    We have developed a method for isolating goldfish microglia. Cells were identified as microglia immunohistochemically with NN-2, a monoclonal antibody (MAb) raised against teleost retinal microglial cells, and by their phagocytic abilities. Morphological characterization of the cells identified round, phase-bright cells as well as flattened macrophage-like cells. Ramified cells were also seen but they were rare. Fusion of macrophage-like cells occurred in high density cultures and resulted in the formation of giant cells that disintegrated a few days later. Immunohistochemical studies demonstrated that virtually all of the cells in our cultures were NN-2+ and did not label with either antiGFAP (an astrocyte marker) or MAb 6D2 (an oligodendrocyte marker). Cells identified as microglia were intensely phagocytic and ingested latex microspheres, DiIAcLDL and goldfish myelin in vitro. In addition, we labelled microglial cells in vivo with intracranial injections of fluorescent dextran and found that microglia isolated from these animals contained the dextran and phagocytosed microspheres. We also studied the effect of myelin on microsphere uptake and compared the effect of myelin and opsonized myelin on the phagocytic activity of the cells. Our results showed a clear increase in the phagocytic activity of microglia when incubated with myelin, with an enhanced effect of opsonized myelin.

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Copyright © 1984 Published by Elsevier Ltd.

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